Journal: Pediatric research

Article Title: Galectin-3 Expression and Effect of Supplementation in Neonatal Mice with Disseminated Candida albicans Infection

doi: 10.1038/s41390-019-0279-x

Figure Lengend Snippet: Tissue fungal burden and galectin-3 concentrations in adult mice with disseminated candidiasis. Mice (n=7) were infected via tail-vein injection with C. albicans and euthanized at 48 h after injection. Panel A: Tissue fungal burden. Panel B: Mean gal3 concentration in tissue homogenates compared to animals receiving saline (n=10). Error bars represent standard deviation. No differences in tissue gal3 concentration were detected by ANOVA with inter-group comparisons by the Student-Newman-Keuls Method.

Article Snippet: In a subsequent experiment, pups were delivered and randomized to receive 5 μg carrier-free recombinant mouse gal3 (R&D Systems, Minneapolis, MN) or saline in 20 μl i.p. injection prior to infection with C. albicans as described above.

Techniques: Infection, Injection, Concentration Assay, Saline, Standard Deviation

Journal: Pediatric research

Article Title: Galectin-3 Expression and Effect of Supplementation in Neonatal Mice with Disseminated Candida albicans Infection

doi: 10.1038/s41390-019-0279-x

Figure Lengend Snippet: Tissue fungal burden and galectin-3 concentrations in neonatal mice with disseminated candidiasis. Two-day-old mouse pups (n=14) were infected via intraperitoneal injection with C. albicans and euthanized at 24 h after injection. Panel A: Tissue fungal burden. Panel B: Mean gal3 concentration in tissue homogenates compared to pups receiving saline (n=14). Error bars represent standard deviation. Mean gal3 concentration was reduced in the spleen of infected pups relative to controls based on ANOVA with inter-group comparisons by the Student-Newman-Keuls Method (p=0.04).

Article Snippet: In a subsequent experiment, pups were delivered and randomized to receive 5 μg carrier-free recombinant mouse gal3 (R&D Systems, Minneapolis, MN) or saline in 20 μl i.p. injection prior to infection with C. albicans as described above.

Techniques: Infection, Injection, Concentration Assay, Saline, Standard Deviation

Journal: Pediatric research

Article Title: Galectin-3 Expression and Effect of Supplementation in Neonatal Mice with Disseminated Candida albicans Infection

doi: 10.1038/s41390-019-0279-x

Figure Lengend Snippet: Survival curve of neonatal mice with disseminated candidiasis after pretreatment with recombinant galectin-3. Two-day-old mouse pups were given intraperitoneal injections of either saline or recombinant gal3, 2 h prior to infection with C. albicans . Uninfected pups receiving gal3 only were included as a control. Pretreatment with gal3 reduced mortality in infected compared to saline treated pups based on log-rank test (p=0.02).

Article Snippet: In a subsequent experiment, pups were delivered and randomized to receive 5 μg carrier-free recombinant mouse gal3 (R&D Systems, Minneapolis, MN) or saline in 20 μl i.p. injection prior to infection with C. albicans as described above.

Techniques: Recombinant, Saline, Infection, Control

Journal: Pediatric research

Article Title: Galectin-3 Expression and Effect of Supplementation in Neonatal Mice with Disseminated Candida albicans Infection

doi: 10.1038/s41390-019-0279-x

Figure Lengend Snippet: Tissue fungal burden in neonatal mice with disseminated candidiasis pretreated with galectin-3. Two-day-old mouse pups were given intraperitoneal injections of either saline or recombinant gal3, 2 h prior to infection with C. albicans (n=10 pups per group). Tissues were collected at the time of death or at 72 h in surviving animals. Fungal burden is depicted with the bars representing median values. P values were derived based on analysis using a negative binomial model to account for the variability in these data which are not normally distributed.

Article Snippet: In a subsequent experiment, pups were delivered and randomized to receive 5 μg carrier-free recombinant mouse gal3 (R&D Systems, Minneapolis, MN) or saline in 20 μl i.p. injection prior to infection with C. albicans as described above.

Techniques: Saline, Recombinant, Infection, Derivative Assay

Journal: Pediatric research

Article Title: Galectin-3 Expression and Effect of Supplementation in Neonatal Mice with Disseminated Candida albicans Infection

doi: 10.1038/s41390-019-0279-x

Figure Lengend Snippet: Tissue fungal burden in neonatal mice with disseminated candidiasis at early time points following infection and pretreatment with galectin-3. Two-day-old mouse pups were given intraperitoneal injections of either saline or recombinant gal3, 2 h prior to infection with C. albicans (n=5 pups per group). Pups were euthanized and tissues were collected at 24 and 36 h following infection. Fungal burden is depicted with the bars representing median values. P values were derived based on analysis using a negative binomial model to account for the variability in these data which are not normally distributed. NS – not significant.

Article Snippet: In a subsequent experiment, pups were delivered and randomized to receive 5 μg carrier-free recombinant mouse gal3 (R&D Systems, Minneapolis, MN) or saline in 20 μl i.p. injection prior to infection with C. albicans as described above.

Techniques: Infection, Saline, Recombinant, Derivative Assay

Journal: Upsala Journal of Medical Sciences

Article Title: Surface glycans contribute to differences between seminal prostasomes from normozoospermic and oligozoospermic men

doi: 10.1080/03009734.2019.1592266

Figure Lengend Snippet: Surface glycosylation of seminal prostasomes: lectin- and immune-transmission electron microscopy. A: Lectin-TEM using SNA. Inserts show enlarged characteristic pattern of SNA-reactivity to each sample group. B: Lectin-TEM using ConA. Inserts show enlarged characteristic pattern of ConA-reactivity to vesicles in each sample group. In O, staining of some proteinaceous material was also observed (arrowheads). C: Immune-TEM using anti-galectin-3 antibodies. Inserts show enlarged characteristic pattern of anti-gal-3-reactivity to each sample group. Micrographs show most characteristic patterns obtained. Although differences in the reactivity of particular vesicles could be noticed, it does not affect the general reactivity of the sample (as in IEC when taking all vesicles into account). (N = seminal prostasomes from normozoospermic men; O = seminal prostasomes from oligozoospermic men).

Article Snippet: Monoclonal anti-CD63 antibody (clone TS63) was from Abcam (Cambridge, UK) and biotinylated goat anti-galectin-3 (gal-3) antibodies from R&D Systems (Minneapolis, MN, USA); 3,3′,5,5′-tetramethylbenzidine (TMB), bovine serum albumin (BSA), D-lactose, and methyl- alpha D-mannopyranoside were from Sigma (St. Louis, MO, USA).

Techniques: Transmission Assay, Electron Microscopy, Staining

Journal: Frontiers in Immunology

Article Title: Granulosa cell-derived miR-379-5p regulates macrophage polarization in polycystic ovarian syndrome

doi: 10.3389/fimmu.2023.1104550

Figure Lengend Snippet: Increased M1/M2 ratio and galectin-3 suppresses granulosa cell proliferation in antral, but not preantral follicle stage, as evident in PCOS. (A) PCOS subjects had lower M2 population and higher M1/M2 ratio compared to non-PCOS; (B) PCOS subjects had higher FF content of galectin-1 and -3 compared to non-PCOS; (C) Galectin-1 did not affect rat GC proliferation in preantral and antral follicles, galectin-3 significantly reduced GC proliferation in antral but not preantral follicles; (D) Adding PCOS FF to non-PCOS GCs significantly reduced cell proliferation compared to those treated with non-PCOS FF. However, the effect of PCOS FF on GC proliferation was blocked using galectin-3 neutralizing antibody; (E) Adding human recombinant galectin-3 to non-PCOS GCs significantly suppress proliferation; (F) The combination of galectin-3 and Anti-Müllerian hormone (AMH) improve sensitivity and specificity of PCOS prediction compared to AMH. AMH: AUC=0.96 with sensitivity=0.88 and specificity=0.95 (p<0.0001); Galectin-3: AUC=0.84 with sensitivity=0.65 and specificity=0.95 (p<0.001); Galectin-3+AMH: AUC=0.977 with sensitivity=0.94 and specificity=1 (p<0.0001). Results are expressed as mean±SEM (n=3 replicates each with 2 rats/group). Data were analyzed by two-way ANOVA and tukey post hoc. ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: To neutralize galectin-3, neutralizing antibody (Miltenyi Biotec; 130-112-969; 20 μl) was added to follicular fluid (200 μl), incubated 30 min in room temperature and then added to granulosa cell cultures.

Techniques: Recombinant

Journal: Frontiers in Immunology

Article Title: Granulosa cell-derived miR-379-5p regulates macrophage polarization in polycystic ovarian syndrome

doi: 10.3389/fimmu.2023.1104550

Figure Lengend Snippet: MiR-379-5p-induced M1 polarization regulates granulosa cell proliferation in a follicular stage-specific manner by increasing pro-inflammatory cytokine level. (A) Conditioned media from macrophages transfected by miR379 mimic (CM-mimic) increases aromatase content without affecting proliferation in preantral follicle GC, while it reduces aromatase and proliferation in that of antral follicles. The aromatase and proliferative responses were not significantly influenced by the presence of the CM-inhibitor compared to control in both follicle stages; (B) Profile of cytokines (Proteome Profiler Rat XL Cytokine Array; R&D Systems) in macrophage condition medium indicates that CM-mimic had higher content of inflammatory cytokines, including Cystatin C (5.4 fold), Galectin-3 (5.3 fold), TNFRS11B (4.3 fold), PAI-1 (3.9 fold) and Osteopontin (3.7 fold). In addition, Endostatin, Fibulin 3, TNF-α, Galectin-1, IL-6, Jagged 1 and MMP3 were specific cytokines to CM-mimic. Results are expressed as mean±SEM (n=3 replicates each with 2 rats/group). Data were analyzed by two-way ANOVA and tukey post hoc. * P <0.05, *** P <0.001.

Article Snippet: To neutralize galectin-3, neutralizing antibody (Miltenyi Biotec; 130-112-969; 20 μl) was added to follicular fluid (200 μl), incubated 30 min in room temperature and then added to granulosa cell cultures.

Techniques: Transfection

Journal: Frontiers in Immunology

Article Title: Granulosa cell-derived miR-379-5p regulates macrophage polarization in polycystic ovarian syndrome

doi: 10.3389/fimmu.2023.1104550

Figure Lengend Snippet: Hypothetical model illustrating that uptake of GC-derived exosomal miR-379-5p by macrophages increases M1 and macrophage release of galectin-3, a response which inhibits GC proliferation in antral but not preantral follicles. Androgen induces GC exosomal miR379 release from preantral but not antral follicles. The uptake of GC-derived exosomal miR379 suppresses PDK1 in macrophages, a cellular mechanism shifting macrophage polarization towards M1. M1 macrophage secretes higher level of galectin-3 in FF, a inflammatory cytokine that specifically suppressed GC proliferation in antral but not preantral follicles. (Created in BioRender.com ).

Article Snippet: To neutralize galectin-3, neutralizing antibody (Miltenyi Biotec; 130-112-969; 20 μl) was added to follicular fluid (200 μl), incubated 30 min in room temperature and then added to granulosa cell cultures.

Techniques: Derivative Assay